Monday, May 02, 2016

Honey Protects DNA from Damage

Organic honey supplementation reverses pesticide-induced genotoxicity by modulating dna damage response

Mol Nutr Food Res. 2016 Apr 30

SCOPE:

Glyphosate (GLY), and organophosphorus (OP) insecticides such as chlorpyrifos (CPF) may cause DNA damage and cancer in exposed individuals through mitochondrial dysfunction. Polyphenols ubiquitously present in fruits and vegetables, have been viewed as antioxidant molecules, but also influence mitochondrial homeostasis. Here, honey containing polyphenol compounds was evaluated for its potential protective effect on pesticide-induced genotoxicity.

METHODS AND RESULTS:

Honey extracts from four floral organic sources were evaluated for their polyphenol content, antioxidant activity and potential protective effects on pesticide-related mitochondrial destabilization, ROSN formation and DNA damage response in human bronchial epithelial and neuronal cells. The protective effect of honey was, then evaluated in a residential population chronically exposed to pesticides. The four honey types showed a different polyphenol profile associated with a different antioxidant power. The pesticide-induced mitochondrial dysfunction parallels ROS formation from mitochondria (mtROS) and consequent DNA damage. Honey extracts efficiently inhibited pesticide-induced mtROS formation, and reduced DNA damage by upregulation of DNA repair through NFR2. Honey supplementation enhanced DNA repair activity in a residential population chronically exposed to pesticides, which resulted in a marked reduction of pesticide-induced DNA lesions.

CONCLUSION:

These results provide new insight regarding the effect of honey containing polyphenols on pesticide-induced DNA damage response.

Evaluation of a potentially probiotic non-dairy beverage developed with honey and kefir grains: Fermentation kinetics and storage study

Food Sci Technol Int. 2016 Apr 26

The aim of this work was to study the fermentation process of honey with kefir grains through a comprehensive understanding of its rheological properties, probiotic cell viability, instrumental color parameters and kinetic aspects in a batch bioreactor and during storage.

The results showed that kefir grains were well adapted to bioreactor conditions, reaching high levels of cell viability (over 106 CFU mL-1 for total yeast and bacteria), phenolic compounds content (190 GAE/100 g) and acidification after 24 h of fermentation at 30 ℃. Colorimetric analysis showed that lightness (L*) and redness (a*) remained constant, while yellowness intensities (b*) decreased during fermentation time. After 35 days of storage, honey kefir beverage maintained its chemical characteristics and microbial viability as required to be classified as a probiotic product. The Ostwald-de-Waele (R2 ≥ 0.98) and Herschel-Bulkley (R2 ≥ 0.99) models can be used to predict the behavior of honey kefir beverage.

The parameters analyzed in this study should be taken into account for industrial production of this novel non-dairy beverage.

Sunday, May 01, 2016

New Zealand Kanuka Honey Treats Rosacea, Acne, Cold Sores, Burns and Wounds.


Honeylab medical‐grade kanuka honey a vanguard for pharmaceutical honey industry

Stuff, 5/1/2016

A medical honey developed by New Zealand researchers can not only treat a number of skin diseases, but could so help combat the global health emergency of antibiotic resistance, creating a billion dollar honey industry.

The medical‐grade kanuka honey formulation developed by Wellington and Bay of Plenty based Honeylab, along with researchers from the Medical Research Institute of New Zealand, are vying with manuka to be a new medical miracle and a major new earner for the Kiwi economy.

The current size of the potential kanuka market is estimated to be about $3 billion and the skin medicines are showing such positive results that several of the top 10 largest healthcare companies in the world are in negotiations with Honeylab for the products...

Saturday, April 30, 2016

Malaysian Tualang Honey Protects Against Memory Loss in Ageing

Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats

Oxid Med Cell Longev. 2016;2016:1549158

Ageing and stress exposure may lead to memory impairment while oxidative stress is thought to be one of the underlying mechanisms involved. This study aimed to investigate the potential protective effects of Tualang honey supplementation on memory performance in aged rats exposed to noise stress.

Tualang honey supplementation was given orally, 200 mg/kg body weight for 28 days. Rats in the stress group were subjected to loud noise, 100 dB(A), 4 hours daily for 14 days. All rats were subjected to novel object recognition test for evaluation of memory performance. It was observed that the rats subjected to noise stress exhibited significantly lower memory performance and higher oxidative stress as evident by elevated malondialdehyde and protein carbonyl levels and reduction of antioxidant enzymes activities compared to the nonstressed rats.

Tualang honey supplementation was able to improve memory performance, decrease oxidative stress levels, increase brain-derived neurotrophic factor (BDNF) concentration, decrease acetylcholinesterase activity, and enhance neuronal proliferation in the medial prefrontal cortex (mPFC) and hippocampus.

In conclusion, Tualang honey protects against memory decline due to stress exposure and/or ageing via enhancement of mPFC and hippocampal morphology possibly secondary to reduction in brain oxidative stress and/or upregulation of BDNF concentration and cholinergic system.

Friday, April 29, 2016

Apitherapy Popular in Saudi Arabia

Saudi beekeepers need specialized training in apitherapy

Saudi Gazette, 4.27.2016

JEDDAH – Honey and beehive products can cure up to 500 diseases and even prevent thousands. Moreover, training in Saudi Arabia is needed to produce professional beekeepers as well as physicians practicing alternative medicine using honey, according to Professor Ahmad Al-Ghamdi, supervisor of the Abdullah Bugshan Chair for Bee Research at King Saud University and chairman of Beekeepers Cooperative Association (BCA), who spoke to Saudi Gazette on the sidelines of the 13th Asian Apicultural Association Conference.

The 3-day conference, which concluded on Tuesday, was organized in an Arab member country for the first time by the Bee Research Chair of King Saud University.

“Our goal is to gather practicing physicians to present the scientific facts,” Prof. Alghamdi said. “Saudi production is relatively weak compared to consumption. We import 14,000 tons annually. One of the problems we have is that despite the big number of beekeepers, approximately over 5,000, the trained professionals may not even reach 1%.”

He added the BCA aims to focus on training at an official institute specialized in training beekeepers.

When asked about Saudis’ receptiveness toward alternative medicine, particularly apitherapy, he said it was favorable. He said “people usually prefer natural products especially because they don’t have side effects.”

Under the theme of “Natural Resource Conservation for Sustainable Development”, leading doctors and apitherapists from 35 countries presented various subjects related to bees, products, technology, and obstacles of beekeeping in climate, care, among others. Activities and workshops were attended by Saudi beekeepers and physicians. Over 100 exhibitors both locally and internationally from countries including Turkey, China, Germany, the United States, participated at the conference.

Dr. Stefan Stangaciu, president of several organizations and associations, including the Apitherapy Consulting & Trading International Ltd., the German Apitherapy Society, and the Romanian Apitherapy Society, displayed the latest scientific practices and products in apitherapy. Speaking to Saudi Gazette, he said beehive products are important to prevent hundreds and thousands of diseases. Secondly, it can treat between 800 and 1,000 diseases according to scientific literature...

Wednesday, April 27, 2016

Montreal Apitherapy Conference May 5-9


From May 05 to May 09, 2016, at the Concordia University of Montreal

10 Reasons why you should go to the Montreal Apitherapy Conference:

1. To participate into the first Apitherapy Conference following the creation of the Canadian Apitherapy Association.
2. To get the most out of your Canadian Apitherapy Association Membership, become a member or get involved in our work groups.
3. To understand the connections between apitherapy, Traditional Chinese Medicine and other alternative medicines.
4. To attend the largest Apitherapy Conference to date in Canada held in both french and english.
​5. To attend a congress led by the most renowned apitherapy doctor in Europe: Dr Stefan Stangaciu.
6. To learn cutting edge methods using bee products in everyday life.
7. To access a professional network: newsletter, forum and more.
8. To understand how we can help bees, biodiversity and organic farming through ensuring a high level of quality bee products for apitherapy across Canada.
9. To visit, shop or become a vendor in our 5 days Api-Expo.
10. To be a pioneer in the establishment of a new alternative medicine in Canada, a promise for faster healing of certain diseases.

Our Speakers:

Dr Stefan Stangaciu

Marie-Pierre Fortier

Adrien Thibault

Yann Loranger

Tuesday, April 26, 2016

Bee Pollen May Help Treat Autistic Children Suffering from Detoxification Deficiencies, Chronic Inflammation and Abnormal Gut Microbiota


Therapeutic potency of bee pollen against biochemical autistic features induced through acute and sub-acute neurotoxicity of orally administered propionic acid

BMC Complement Altern Med. 2016 Apr 23;16(1):120

BACKGROUND:

It is now well documented that postnatal exposure to certain chemicals has been reported to increase the risk of autism spectrum disorder. Propionic acid (PA), as a metabolic product of gut microbiotaandas a commonly used food additive, has been reported to mediate the effects of autism. Results from animal studies may help to identify environmental neurotoxic agents and drugs that can ameliorate neurotoxicity and may thereby aid in the treatment of autism. The present study investigated the ameliorative effects of natural bee pollen against acute and sub-acute brain intoxication induced by (PA) in rats.

METHODS:

Twenty-four young male Western Albino ratswere enrolled in the present study. They were classified into four equal groups, eachwith6 rats. The control group received only phosphate buffered saline; the oral buffered PA-treated groups (II and III) received a neurotoxic dose of 750 mg/kg body weight divided in 3 dose of 250 mg/kg body weight/day serving asthe acute group and 750 mg/kg body weight divided in 10 equal dose of 75 mg/kg body weight/day as the sub-acute group. The fourth group received 50 mg bee pollen for 30 days after PA-acute intoxication.

RESULTS:

The obtained data showed that the PA-treated groups demonstrated multiple signs of brain toxicity, as indicated by a depletion of serotonin (5HT), dopamine and nor-adrenaline, together withan increase in IFN-γ and caspase 3. Bee pollen was effective in ameliorating the neurotoxic effect of PA. All measured parameters demonstrated minimal alteration in comparison with thecontrol animal than did those of acute and sub-acute PA-treated animals.

CONCLUSIONS:

In conclusion, bee pollen demonstrates anti-inflammatory and anti-apoptotic effects while ameliorating the impaired neurochemistry of PA-intoxicated rats.

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Based upon the results of the present study, bee pollen can be suggested as a treatment strategy for autistic children that suffer from detoxification deficiencies, demonstrated chronic inflammation and abnormal gut microbiota.

Monday, April 25, 2016

Manuka honey, one of nature’s unique wonder foods

It has been used successfully in treatment of infections caused by antibiotic-resistant micro-organisms.

Indian Express, 4/23/2016

Honey is more than just a natural sweetener. Historically, it is known to have medicinal properties. In general, most honeys possess anti-bacterial, anti-oxidant, anti-inflammatory and prebiotic properties.
Other benefits that have been attributed to honey include anti-tumour, anti-mutagenic and anti-viral properties dependent on the nutritional composition of the honey consumed. It is well known for its wound healing properties and as a gastro-intestinal remedy since ancient times.

Several types of honey are available, each with a distinct taste and properties.

The broad spectrum anti-bacterial activity of honey is attributed to various factors including its high sugar concentration (80 per cent sugar) and hydrogen peroxide.

Certain honeys from specific plants are more effective than others. Manuka honey that comes from New Zealand Manuka bush is one such example. It has been found to be an effective therapy for digestive complaints ranging from acid reflux, gastritis, diarrhea, ulcers and fungal infections. It has been used in upper respiratory infections including chronic sinusitis, rhinitis and allergic symptoms...

Sunday, April 24, 2016

Propolis May Help Prevent Heart Disease

Turkish propolis protects human endothelial cells in vitro from homocysteine-induced apoptosis

Acta Histochem. 2016 Apr 13. pii: S0065-1281(16)30041-1

Chronic cardiovascular and neurodegenerative complications induced by hyperhomocysteinemia have been most relatively associated with endothelial cell injury. Elevated homocysteine (Hcy) generates reactive oxygen species (ROS) accompanying with oxidative stress which is hallmarks of the molecular mechanisms responsible for cardiovascular disease.

Propolis is a natural product, obtained by honeybee from various oils, pollens, special resins and wax materials, conventionally used with the purpose of treatment by folks Propolis has various biological activities and powerful antioxidant capacity. The flavonoids and phenolic acids, most bioactive components of propolis, have superior antioxidant ability to defend cell from free radicals.

This study was designed to examine the protective effects of Turkish propolis (from east of country) on Hcy induced ROS production and apoptosis in human vascular endothelial cells (HUVECs).

According to results, co-treatment of HUVECs with propolis decreased Hcy-induced ROS overproduction and lipid peroxidation (LPO) levels. Furthermore, overproductions of Bax, caspase-9 and caspase-3 protein, elevation of cytochrome c release in Hcy-treated HUVECs were significantly reduced by propolis.

It was concluded that propolis has cytoprotective ability against cytotoxic effects of high Hcy in HUVECs.

Saturday, April 23, 2016

Bee Venom Helps People Suffering from HIV

Apitoxin from Bee Venom Helps People Suffering from HIV – Alternative Antiretroviral Drug?

Science News Hub, April 19, 2016

MEXICO – The University of Guadalajara (UDG) found, in a recent  research study, that bee venom known as apitoxin helps the immune system of people with Human Immunodeficiency Virus (HIV) that causes AIDS.

In an interview with the News Agency of the National Council of Science and Technology (CONACYT), the researcher Sergio Alvarez Barajas explained that they worked with a group of 28 infected people who willingly cooperate in the investigation.  Everyone who was participated in the research had to provide a document copy that guarantees that one is HIV positive and have their first clinical results with levels of CD4 and CD8 analysis.

Barajas said that they did a poison tolerance test, and a solution with a certain concentration of poison, where each patient took 15 drops a day, five before each meal.

CD4 and CD8 cells are types of lymphocytes that are part of the human’s immune system.  Sick people with AIDS or HIV positive are normally deficient in the number of CD4 while CD8 is increased.

The researcher said that is why the measurement of these cells helps doctors determine the mechanisms of retroviral in patient and disease progression.

The results in the laboratory study group showed an increase in the number of CD4, with respect to the results of the first analysis, and decreased CD8, which is favorable compared to retroviral treatments, said the biologist...

Friday, April 22, 2016

NPR Podcast: How to Use Products of the Bee Hive for Healing

NPR, 4/14/2016

You may not think of bees as domestic animals, but any beekeeper would set you straight. Bees range far and wide when they forage for nectar and pollen, but they return home to the bee hive. Products of the hive have long been used for healing.

Healing with Products of the Bee Hive: We get updates on bee venom therapy, also known as apipuncture. We hear how bee stings can be used to ease the hard-to-treat pain of post-herpetic neuralgia. Our guest experts also describe how to use other products of the bee hive such as honey, propolis and royal jelly from the complementary perspectives of a physician and a beekeeper.

This Week's Guests: Andrew Kochan, MD, practices physical medicine, rehabilitation and prolotherapy in Los Angeles. He is director of the Institute for Healing Arts Research and past president of the American Apitherapy Society.

Thursday, April 21, 2016

Propolis May Be Used in Root Canals

Application of chitosan and propolis in endodontic treatment: a review

Mini Rev Med Chem. 2016 Apr 18

Chitosan and propolis are natural substances that can be commonly found in the environment. Chitosan is one of several cationic polysaccharides found in nature. It is a natural biopolymer transformed from chitin which is a product of crustacean shells.

Propolis is produced by honeybees through mixing the secretions of their hypopharyngeal glands with the digested product of resins collected from plants. Due to their excellent chemical and biological properties, chitosan and propolis arouse keen interest in dental science, including endodontics.

There are various possibilities for applying propolis and chitosan based medicaments in endodontic treatment of root canals, and what is particularly emphasized is their potential effectiveness against resistant microorganisms such as E. faecalis and C. albicans, as well as biocompatible to the periapical tissues in comparison with the most commonly used agents They aim at microflora that is difficult to eliminate, and their medicinal properties, including anti-inflammatory, antifungal and antiseptic properties, make them suitable for use in endodontic treatment.

Due to potential side effects of commonly used synthetic drugs and other safety related reasons, natural alternatives for endodontic usage are continuously explored and tested. The paper presents the possibilities for applying propolis and chitosan in endodontic treatment on the basis of chosen articles published in recent years.

Wednesday, April 20, 2016

Study of Bee Venom Immuno-Modulatory Effect

Effect of Bee Venom and Its Fractions on the Release of Pro-Inflammatory Cytokines in PMA-Differentiated U937 Cells Co-Stimulated with LPS

Vaccines 2016, 4(2), 11, Published: 19 April

The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed.

The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS.

Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol.

The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.

Tuesday, April 19, 2016

Melittin, the Major Pain-Producing Substance of Bee Venom

Neuroscience Bulletin
pp 1-8
First online: 17 March 2016

Melittin is a basic 26-amino-acid polypeptide that constitutes 40–60% of dry honeybee (Apis mellifera) venom. Although much is known about its strong surface activity on lipid membranes, less is known about its pain-producing effects in the nervous system.

In this review, we provide lines of accumulating evidence to support the hypothesis that melittin is the major pain-producing substance of bee venom. At the psychophysical and behavioral levels, subcutaneous injection of melittin causes tonic pain sensation and pain-related behaviors in both humans and animals. At the cellular level, melittin activates primary nociceptor cells through direct and indirect effects. On one hand, melittin can selectively open thermal nociceptor transient receptor potential vanilloid receptor channels via phospholipase A2-lipoxygenase/cyclooxygenase metabolites, leading to depolarization of primary nociceptor cells. On the other hand, algogens and inflammatory/pro-inflammatory mediators released from the tissue matrix by melittin’s pore-forming effects can activate primary nociceptor cells through both ligand-gated receptor channels and the G-protein-coupled receptor-mediated opening of transient receptor potential canonical channels. Moreover, subcutaneous melittin up-regulates Nav1.8 and Nav1.9 subunits, resulting in the enhancement of tetrodotoxin-resistant Na+ currents and the generation of long-term action potential firing.

These nociceptive responses in the periphery finally activate and sensitize the spinal dorsal horn pain-signaling neurons, resulting in spontaneous nociceptive paw flinches and pain hypersensitivity to thermal and mechanical stimuli.

Taken together, it is concluded that melittin is the major pain-producing substance of bee venom, by which peripheral persistent pain and hyperalgesia (or allodynia), primary nociceptive neuronal sensitization, and CNS synaptic plasticity (or metaplasticity) can be readily induced and the molecular and cellular mechanisms underlying naturally-occurring venomous biotoxins can be experimentally unraveled.