Tuesday, February 09, 2016

AUDIO: Manuka Honey Replaces Antibiotics in Hospitals


Three Australian universities have teamed up to explore the eighty species of Manuka that could transform the local honey industry from just food to medical saviours.

Primarily produced in New Zealand, Manuka is so effective at preventing infection it's now in high demand in hospitals where anti-biotic resistant bacteria like staphylococcus plays havoc with patient health.

Monday, February 08, 2016

Australian Producers Set to Tap Global Market for Medicinal Honey

Liquid gold rush beckons for Australian honey producers as research identifies best antimicrobial nectar

ABC, 2/5/2016

Australian honey producers are set to tap into a potential billion-dollar global market for medicinal honey, with new research confirming powerful antimicrobial properties in the flowering nectar of trees found across Australia.

The joint study by three Australian universities is testing up to 86 different species of Leptospermum, 10 times more than are found across the Tasman, where the trees are the basis of New Zealand's burgeoning manuka honey industry.

Sunday, February 07, 2016

Therapeutic Potential of Brazilian Red Propolis

A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases

Eur J Med Chem. 2016 Jan 20;110:267-279

Propolis is a complex resinous mixture collected by bees, with high medicinal, historical and economic value. The nutraceutical and pharmacological benefits of propolis have been extensively explored in several fields of medicine as an important resource for prevention and treatment of oral and systemic diseases.

A relatively new type of propolis, named red propolis (in Brazil, Brazilian Red Propolis - BRP), has been arousing attention for the promising pharmacological properties of some of its isolated compounds (vestitol, neovestitol, quercetin, medicarpin, formononetin, etc). Due to a distinct chemical composition, BRP and its isolated compounds (mainly isoflavones) affect a wide range of biological targets and could have an impact against numerous diseases as an antimicrobial, anti-inflammatory and immunomodulatory, antioxidant and antiproliferative agent.

In this review, we comprehensively address the main aspects related to BRP bioprospection, chemistry and therapeutic potential. Further information is provided on mechanisms of action discovered thus far as well as clinical use in humans and regulatory aspects. As of now, BRP and its isolated molecules remain a fascinating topic for further research and application in biomedical areas and dentistry.

Saturday, February 06, 2016

Korean Bee Pollen Shows Neuraminidase Inhibitory Activity

Characterization of Neuraminidase Inhibitors in Korean Papaver rhoeas Bee Pollen Contributing to Anti-Influenza Activities In Vitro

Planta Med. 2016 Feb 5

The active constituents of Korean Papaver rhoeas bee pollen conferring neuraminidase inhibitory activities (H1N1, H3N2, and H5N1) were investigated. Six flavonoids and one alkaloid were isolated and characterized by nuclear magnetic resonance and mass spectrometry data. These included kaempferol-3-sophoroside (1), kaempferol-3-neohesperidoside (2), kaempferol-3-sambubioside (3), kaempferol-3-glucoside (4), quercetin-3-sophoroside (5), luteolin (6), and chelianthifoline (7).

All compounds showed neuraminidase inhibitory activities with IC50 values ranging from 10.7 to 151.1 µM. The most potent neuraminidase inhibitor was luteolin, which was the dominant content in the ethyl acetate fraction. All tested compounds displayed noncompetitive inhibition of H3N2 neuraminidase. Furthermore, compounds 1-7 all reduced the severity of virally induced cytopathic effects as determined by the Madin-Darby canine kidney cell-based assay showing antiviral activity with IC50 values ranging from 10.7 to 33.4 µM (zanamivir: 58.3 µM).

The active compounds were quantified by high-performance liquid chromatography, and the total amount of compounds 1-7 made up about 0.592 g/100 g bee pollen, contributing a rich resource of a natural antiviral product.

Thursday, February 04, 2016

Flavonoid Present in Honey and Propolis Improves Glycemic Control

Role of chrysin on expression of insulin signaling molecules

J Ayurveda Integr Med. 2015 Oct-Dec;6(4):248-58


Currently available drugs are unsuccessful for the treatment of tye-2 diabetes due to their adverseside-effects. Hence, a search for novel drugs, especially ofplant origin, continues. Chrysin (5,7-dihydroxyflavone) is a flavonoid, natural component of traditional medicinal herbs, present in honey, propolis and many plant extracts that hasbeen used in traditional medicine around the world to treat numerous ailments.


The present study was aimed to identify the protective role of chrysin on the expression of insulin-signaling molecules in the skeletal muscle of high fat and sucrose-induced type-2 diabetic adult male rats.


The oral effective dose of chrysin (100 mg/kg body weight) was given once a day until the end of the study (30 days post-induction of diabetes) to high fat diet-induced diabetic rats. At the end of the experimental period, fasting blood glucose, oral glucose tolerance, serum lipid profile, lipid peroxidation (LPO) and free radical generation, as well as the levels of insulin signaling molecules and tissue glycogen in the gastrocnemius muscle were assessed.


Diabetic rats showed impaired glucose tolerance and impairment in insulin signaling molecules (IR, IRS-1, p-IRS-1Tyr(632), p- Akt(Thr308)), glucose transporter subtype 4 [GLUT4] proteins and glycogen concentration. Serum insulin, lipid profile, LPO and free radical generation were found to be increased in diabetic control rats. The treatment with chrysin normalized the altered levels of blood glucose, serum insulin, lipid profile, LPO and insulin signaling molecules as well as GLUT4 proteins.


Our present findings indicate that chrysin improves glycemic control through activation of insulin signal transduction in the gastrocnemius muscle of high fat and sucrose-induced type-2 diabetic male rats.

Wednesday, February 03, 2016

Water-Soluble Royal Jelly May Help Treat Skin Pigmentation

Whitening Effect of Watersoluble Royal Jelly from South Korea

Korean J Food Sci Anim Resour. 2015;35(5):707-13

Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein.

In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting.

WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes.

Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.

Tuesday, February 02, 2016

Propolis had Significant Antifungal Activity

Antifungal Activity of Propolis Against Yeasts Isolated From Blood Culture: In Vitro Evaluation

J Clin Lab Anal. 2016 Jan 20


Due to the failure of available antifungal agents in the treatment of candidemia and the toxic activities of these drugs, a lot of researches are being conducted to develop new nontoxic and effective antifungal agents for optimal control of fungal pathogens. The aim of this study is to evaluate the in vitro antifungal activity of propolis against yeasts isolated from the blood cultures of intensive care unit patients.


Seventy-six strains were included in this study. The in vitro antifungal activity of propolis, fluconazole (FLU), and itraconazole (ITR) was investigated by the microdilution broth methods (CLSI guidelines M27-A3 for yeast). The propolis sample was collected from Kayseri, Turkey.


Of the 76 isolates, 33 were identified as Candida albicans while 37 were C. parapsilosis, three were C. tropicalis, and three were identified as C. glabrata. The geometric mean range for MIC (μg/ml) with regard to all isolates was 0.077 to 3 μg/ml for FLU and ITR, and 0.375 to 0.70 μg/ml for propolis. It was shown that propolis had significant antifungal activity against all Candida strains and the MIC range of propolis was determined as 0185 to 3 μg/ml.


This study demonstrated that propolis had significant antifungal activity against yeasts isolated from blood culture compared with FLU and ITR. The propolis MIC in azole-resistant strains such as C. glabrata was found lower than the FLU MIC.

Monday, February 01, 2016

Bee Venom Boosts Wound Healing

Bee Venom Accelerates Wound Healing in Diabetic Mice by Suppressing Activating Transcription Factor-3 (ATF-3) and Inducible Nitric Oxide Synthase (iNOS)-Mediated Oxidative Stress and Recruiting Bone Marrow-Derived Endothelial Progenitor Cells

J Cell Physiol. 2016 Jan 30

Multiple mechanisms contribute to impaired diabetic wound healing including impaired neovascularization and deficient endothelial progenitor cell (EPC) recruitment. Bee venom (BV) has been used as an anti-inflammatory agent for the treatment of several diseases. Nevertheless, the effect of BV on the healing of diabetic wounds has not been studied.

Therefore, in this study, we investigated the impact of BV on diabetic wound closure in a type I diabetic mouse model. Three experimental groups were used: group 1, non-diabetic control mice; group 2, diabetic mice; and group 3, diabetic mice treated with BV. We found that the diabetic mice exhibited delayed wound closure characterized by a significant decrease in collagen production and prolonged elevation of inflammatory cytokines levels in wounded tissue compared to control non-diabetic mice. Additionally, wounded tissue in diabetic mice revealed aberrantly up-regulated expression of ATF-3 and iNOS followed by a marked elevation in free radical levels. Impaired diabetic wound healing was also characterized by a significant elevation in caspase-3, -8 and -9 activity and a marked reduction in the expression of TGF-β and VEGF, which led to decreased neovascularization and angiogenesis of the injured tissue by impairing EPC mobilization. Interestingly,

BV treatment significantly enhanced wound closure in diabetic mice by increasing collagen production and restoring the levels of inflammatory cytokines, free radical, TGF-β and VEGF. Most importantly, BV-treated diabetic mice exhibited mobilized long-lived EPCs by inhibiting caspase activity in the wounded tissue. Our findings reveal the molecular mechanisms underlying improved diabetic wound healing and closure following BV treatment.

Sunday, January 31, 2016

Antimicrobial Effect of Manuka Honey Enhanced by Light Exposure

Preliminary evaluation of photodynamic activity of manuka honey

Photodiagnosis Photodyn Ther. 2016 Jan 25


•The phototoxic effect of manuka honey on Pseudomonas aeruginosa was screened.
•In vitro results suggest that antimicrobial effect of manuka honey can be enhanced by light exposure.
•A light dependent reduction of bacteria population was observed.
•A combination of honey and PDT may be an effective treatment for wound infections.

Saturday, January 30, 2016

Bee Venom Acupuncture May Help Treat Dermatitis

Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice

BMC Complementary and Alternative Medicine
Published: 29 January 2016


Bee venom acupuncture (BVA), a novel type of acupuncture therapy in which purified bee venom is injected into the specific acupuncture point on the diseased part of the body, is used primarily for relieving pain and other musculoskeletal symptoms. In the present study, therapeutic potential of BVA to improve atopic dermatitis, a representative allergic dysfunction, was evaluated in the mouse model of trimellitic anhydride (TMA)-induced skin impairment.


Mice were treated with 5 % TMA on the dorsal flank for sensitization and subsequently treated with 2 % TMA on the dorsum of both ears for an additional 12 days after a 3-day interval. From the 7th day of 2 % TMA treatment, bilateral subcutaneous injection of BV (BV, 0.3 mg/kg) was performed daily at BL40 acupuncture points (located behind the knee) 1 h before 2 % TMA treatment for 5 days.


BVA treatment markedly inhibited the expression levels of both T helper cell type 1 (Th1) and Th2 cytokines in ear skin and lymph nodes of TMA-treated mice. Clinical features of AD-like symptoms such as ear skin symptom severity and thickness, inflammation, and lymph node weight were significantly alleviated by BV treatment. BV treatment also inhibited the proliferation and infiltration of T cells, the production of Th1 and Th2 cytokines, and the synthesis of interleukin (IL)-4 and immunoglobulin E (IgE)—typical allergic Th2 responses in blood. The inhibitory effect of BVA was more pronounced at BL40 acupoint than non-acupuncture point located at the base of the tail.


These results indicate that BV injection at specific acupuncture points effectively alleviates AD-like skin lesions by inhibiting inflammatory and allergic responses in a TMA-induced contact hypersensitivity mouse model.

Friday, January 29, 2016

Oman Clinic Offers Apitherapy Treatments

Bee Therapy and Medicinal Honey in Muscat

Times of Oman, 1/28/2016

Did you know that the honeybee has been around for million years and are the only insect that produce food for humans? And did you know that every single thing a bee produces has tremendous medicinal properties? I was unaware of these facts and many other bee-related facts until I met Dr Hassan Talib Al Lawati last month.

I got acquainted with Dr Hassan at the 5th Annual Honey Market. He holds a PhD in Bee Science and works for the Ministry of Agriculture and Fisheries. I was fascinated by his knowledge and enthusiasm about all things related to bees and beekeeping, so I was thrilled when he invited me to his Bee Products Healing Centre to learn more about Apitherapy, an alternate medicinal treatment popular in Europe, UK, Brazil, Italy, Germany, and France, that uses honey and other bee products to heal diseases. Contrary to the popular misconception, apitherapy uses all hive products including, honey, propolis, pollen, royal jelly, and bee wax for healing, not only bee venom, for a wide range of ailments and it has been increasingly gaining popularity and credibility in Oman since its inception in 2009.

Dr Hassan’s Apitherapy Clinic in Al Khuwair exclusively uses bee products to heal various diseases and illnesses. Located on the 7th floor of the Al Amal hospital building, BPHC, it wasn’t difficult to find it as the honey aroma was so strong that I was able to follow the scent from the elevator to the clinic’s doorstep. I stepped over the threshold into a bee wonderland filled with yellow and black striped combinations, bee soft toys, bee wall hanging, animated charts on walls, and a showcase cupboard full of bright coloured syrups, lotions, and cream bottles, capsule strips, soaps, and shampoos. In addition to the standard bee products, like honey and bee wax, he offers therapies that use smell of honey for healing sinus, flu, lung infections, and asthma; bee sound to stimulate the ear drum and improve hearing; and honey massages on the face, back, and feet for cosmetic benefits. A “bee cocktail” mixes all the natural bee products and byproducts to cure weakness and insomnia among other illnesses...

Thursday, January 28, 2016

Manuka Honey Boosts Healing of Horse Wounds

Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full-thickness skin wounds in equine distal limbs

Aust Vet J. 2016 Jan;94(1-2):27-34


To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)-β1 and TGF-β3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds.


In this experimental study of 10 Standardbred horses, five full-thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non-contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF-β1 and TGF-β3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology.


Manuka honey gel had no significant effect on TGF-β1 and TGF-β3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10).


Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.

Wednesday, January 27, 2016

Honey May Help Prevent Colon Cancer

Honey and its Phytochemicals: Plausible Agents in Combating Colon Cancer through its Diversified Actions

Journal of Food Biochemistry

Cancer is one of the leading causes of death worldwide. Among them, colorectal cancer is linked with diet as the epithelial cells of colon and rectum come in direct contact with diet. Diets rich in phenolic constituents are associated in eradicating various noncommunicable diseases including cancer. This work illustrates antiproliferative effects of honey and its phytochemicals against colorectal cancer to limelight. Honey and its phytochemicals are found to inhibit the cancer growth. Changes like activation of caspase-3, caspase-8, polyadenosine diphosphate-ribose polymerase cleavage, boost in ROS level activating mitochondrial pathway, lipid layer breakage, DNA fragmentation, increase of G0/G1 phase cells, up-regulation of Bax regulators and p53 dependent apoptosis are significant. It is high time for scientists to initiate more trails using honey and its phytochemicals against some immune-deficient animal models of colon cancer and in low- and high-risk human individuals to validate honey as a mighty sword against colon cancer. This review promulgates honey and its phytochemicals as candidates in colon cancer prevention.

Practical Application

Colon cancer is the third largest cause of cancer death worldwide. The standard chemotherapeutic agents are usually noneffective in the latter stages of cancer. As colorectal cancer is more prone to the diet consumed, scientists have explored many dietary compounds to treat this particular type of cancer. In our review, we highlight the effect of honey and its phenolic constituents against the colon cancer cells. Various, in vitro experiments done are enlisted. This review also emphasis the need of numerous studies that are needed to be initiated to explore the anticancer effect of crude honey specifically. As honey is one of the ingredients used in daily life, honey can also be used in chemoprevention against colon cancer.

Tuesday, January 26, 2016

Malaysian Tualang Boosts Healing of Bowel Wounds

Effect of Tualang honey on the anastomotic wound healing in large bowel anastomosis in rats-A randomized controlled trial

BMC Complement Altern Med. 2016 Jan 23;16(1):28


Honey has long been used for the treatment of number of ailments and diseases including surgical wounds. Current study evaluates the effectiveness of Tualang honey (TH) for large bowel anastomotic healing in Wistar rats.


Thirty male Wistar rats were given a 3 centimeter infra-umbilical laparotomy wound, in`flicted on their abdomen. The colonic transection was performed at 5 cm distal to caecum, with end to end anastomosis of colon segment. They were divided into two groups. Group I was fed with standard rat chow and water. Meanwhile, Group II apart from standard feed, was also given TH 1.0 g/kg every morning until day seven post operatively. Afterwards, anastomotic bursting pressures were measured and histopathological examination on the anastomosis line was performed with light microscopes. The data from two groups were analyzed by Independent paired t test for continuous variables.


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Oral treatment with TH enhances anastomotic wound healing by increasing the number of fibroblasts and by decreasing inflammatory cells leading towards increased wound strength.

Sunday, January 24, 2016

Propolis Paste Boosts Wound Healing

Effect of Propolis on Experimental Cutaneous Wound Healing in Dogs

Vet Med Int. 2015;2015:672643

This study evaluates clinically the effect of propolis paste on healing of cutaneous wound in dogs.

Under general anesthesia and complete aseptic conditions, two full thickness skin wounds (3 cm diameter) were created in each side of the chest in five dogs, one dorsal and one ventral, with 10 cm between them. These wounds were randomly allocated into two groups, control group (10 wounds) and propolis group (10 wounds). Both groups were represented in each dog. The wounds were cleaned with normal saline solution and dressed with macrogol ointment in control group and propolis paste in propolis group, twice daily till complete wound healing. Measurement of the wound area (cm(2)) was monitored planimetrically at 0, 7, 14, 21, 28, and 35 days after injury. The data were analyzed statistically.

The results revealed a significant reduction in the wound surface area in the propolis group after 14 and 21 days compared to control group. The wound reepithelization, contraction, and total wound healing were faster in propolis group than in control group during five weeks of study.

In conclusion, propolis paste has a positive impact on cutaneous wound healing and it may be suggested for treating various types of wounds in animals.